Summary of Study ST001910

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001204. The data can be accessed directly via it's Project DOI: 10.21228/M82X2K This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001910
Study TitleImpact of host dietary BCAA to gut symbiont-derived lipid profile
Study SummaryWe analyzed mouse feces samples of C57BL/6 mice from diet intervention experiment. Briefly, mice were fed with BCAA-sufficient formulated diet (Testdiet 5CC7) for seven days, followed by BCAA-deficient formulated diet (Testdiet 58ZX) for the following seven days. Fecal sample were collected from mice at baseline (n=11), at day 7 (n=10) and at day 14 (n=10).
Institute
Brigham and Women's Hospital
Last NameSungwhan
First NameOh
Address60 Fenwood Rd, BTM 3016
Emailsungwhan_oh@hms.harvard.edu
Phone6175255009
Submit Date2021-07-17
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2022-01-18
Release Version1
Oh Sungwhan Oh Sungwhan
https://dx.doi.org/10.21228/M82X2K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003107
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Vanquish
Column Agilent Eclipse C18 (75 x 4.6mm, 1.8um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode NEGATIVE
Units count per second

Chromatography:

Chromatography ID:CH002294
Chromatography Summary:Reverse-phase C18
Instrument Name:Thermo Vanquish
Column Name:Agilent Eclipse C18 (75 x 4.6mm, 1.8um)
Column Temperature:40
Flow Gradient:ACN:2-propanol:water=65:25:10 to 10:80:10, 0.05% formic acid
Flow Rate:600uL/min
Solvent A:65% acetonitrile/25% isopropanol/10% water; 0.05% formic acid
Solvent B:10% acetonitrile/80% isopropanol/10% water; 0.05% formic acid
Chromatography Type:Reversed phase
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