Summary of Study ST001271
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000857. The data can be accessed directly via it's Project DOI: 10.21228/M8X110 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001271 |
Study Title | Colorectal cancer before and after surgery metabolomics data integration |
Study Type | Pilot study |
Study Summary | In this study 40 urine samples were analyzed in 5 batches. There are 3 labels: control group volunteers (8 pcs., CG), colorectal cancer patients before surgical operation (20 pcs., pre) and after surgical operation (12 pcs., post). |
Institute | Lomonosov MSU |
Department | Chemistry |
Laboratory | Mass spectrometry |
Last Name | Plyushchenko |
First Name | Ivan |
Address | Leninskie gory, 1/3 |
plyush1993@bk.ru | |
Phone | 89160362200 |
Submit Date | 2019-10-29 |
Analysis Type Detail | LC-MS |
Release Date | 2020-01-13 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002111 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Shimadzu 20AD |
Column | ACQUITY UPLC BEH C18 (1.7 µm, 2.1 mm X 5 mm);Agilent ZORBAX StableBond C18 UHPLC guard (4.6 mm, 1.8 µm) |
MS Type | ESI |
MS instrument type | Other |
MS instrument name | Shimadzu LCMS-IT-TOF |
Ion Mode | POSITIVE |
Units | Area |
Chromatography:
Chromatography ID: | CH001541 |
Chromatography Summary: | Two solvents were used: (A) deionized water with 0,1% formic acid, and (B) MeCN. After a 5 min hold at 5.0% solvent B, elution was performed according to the following conditions: from 5.0% B to 59% B in 30 min; to 95% B in 7 min; keeping constant for 10 min, to 5% B in 3 min, followed by 5 min of equilibration on the initial conditions. The flow rate was 0.3 mL•min-1. The injection volume was 5 μL. |
Chromatography Comments: | Acquity UPLC BEH; 1.7 μm 2.1 × 5 mm UHPLC Guard 3PK Zorbax SB-C18 1.8 μm guard column |
Instrument Name: | Shimadzu 20AD |
Column Name: | ACQUITY UPLC BEH C18 (1.7 µm, 2.1 mm X 5 mm);Agilent ZORBAX StableBond C18 UHPLC guard (4.6 mm, 1.8 µm) |
Column Pressure: | 300-500 bar |
Column Temperature: | 30 |
Flow Gradient: | non-linear |
Flow Rate: | 0.3 ml/min |
Injection Temperature: | 5 |
Internal Standard: | papaverine |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |