Summary of Study ST001511
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001018. The data can be accessed directly via it's Project DOI: 10.21228/M83T2V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001511 |
| Study Title | Symbiotic bacteria-derived organic acids |
| Study Summary | Metabolomic profiles of six bacteria and K. oxytoca were compared to identify candidate metabolites that may prevent B. bassiana infection of D. antiqua. |
| Institute | Qilu University of Technology (Shandong Academy of Sciences) |
| Last Name | Zhou |
| First Name | Fangyuan |
| Address | No. 28789 Jingshidong Road, Jinan, Shandong, 250103, China |
| fangyuan_zhou@163.com | |
| Phone | +8618511761347 |
| Submit Date | 2020-09-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-01-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002504 | AN002505 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Unspecified | Unspecified |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | EI | EI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001831 |
| Chromatography Summary: | All samples from the nine groups were analyzed by a LC-MS system. First, all chromatographic separations were performed using an ultra-performance liquid chromatography (UPLC) system (SCIEX, UK) equipped with an ACQUITY UPLC BEH Amide column (100 mm×2.1 mm, 1.7 µm, Waters, UK) for the reversed-phase separation. The column oven was maintained at 35 °C. The flow rate was 0.4 mL/min and the mobile phase consisted of solvent A (25 mM ammonium acetate) and solvent B (isopropanol: acetonitrile =9:1 + 0.1% formic acid). Gradient elution conditions were set as follows: 0-0.5 min, 95% B; 0.5-9.5 min, 95% to 65% B; 9.5-10.5 min, 65%-40% B; 10.5-12 min, 40% B; 12-12.2 min, 40%-95% B; and 12.2-15 min, 95% B. The injection volume for each sample was 4 µL. |
| Instrument Name: | Unspecified |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 35 |
| Flow Gradient: | 0-0.5 min, 95% B; 0.5-9.5 min, 95% to 65% B; 9.5-10.5 min, 65%-40% B; 10.5-12 min, 40% B; 12-12.2 min, 40%-95% B; and 12.2-15 min, 95% B. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 25 mM ammonium acetate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
