Summary of Study ST001943
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001153. The data can be accessed directly via it's Project DOI: 10.21228/M8P10F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001943 |
| Study Title | Urinary signature of chronic kidney disease in patients with severe obesity by CE-MS |
| Study Type | Human nephropathy in CKD obese patients |
| Study Summary | Urine metabolomic characterization of severe obese patients with and without chronic kidney disease (CKD) by CE-MS. Analysis was performed in patients before and after bariatric surgery. In the present studio, samples obtained from CKD patients with severe obesity before bariatric surgery will be referred as OD (obese disease). Samples obtained from CKD patients with severe obesity after bariatric surgery will be referred as ODBS (Obese disease bariatric surgery). Patients with severe obesity without CKD will be referenced as O (obese) and after BS, OBS (obese bariatric surgery). In healthy group, when results refer to the first void urine samples, the acronym will be Healthy1V, and the acronym for urine samples collected at 24-hour will be Healthy24h. |
| Institute | University Rey Juan Carlos |
| Department | Basics Science of Health |
| Laboratory | LAFEMEX |
| Last Name | Lanzon |
| First Name | Borja |
| Address | Avenida de Atenas S/N |
| borja.lanzon@urjc.es | |
| Phone | 663692554 |
| Submit Date | 2021-08-19 |
| Num Groups | 6 |
| Total Subjects | 27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | CE-MS |
| Release Date | 2021-11-04 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003187 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | EI |
| MS instrument type | CE-TOF-MS |
| MS instrument name | Agilent 6224 TOF |
| Ion Mode | POSITIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH002355 |
| Chromatography Summary: | Analysis were performed in a capillary electrophoresis (CE) system coupled to a TOF-MS analyser (TOF: time-of-flight) purchased from Agilent Technologies (CE: 7100, MS: 6224). In coupling process, steath liquid was supplied by an ISO pump (Agilent 1200) to increase the volatility and compensate the volume for MS through an electrospray source. A fused silica capillary was used to perform metabolite separation (100 cm total length x 50 μm i.d. x 360 μm o.d., provided by Agilent Technologies). Capillary was conditioned with water, BGE and NaOH. Before analysis, capillary was rinsed for 5 min at 950 mbar with BGE and was applied a voltage of 30kV for 10 s to displace BGE ions. After conditioning, sample was loaded applying 50 mbar for 50 s, then, BGE was applied at 100 mbar for 10 s. In the capillary was applied a pressure of 25 mbar and a voltage of 30 kV to achieve metabolite separation. A positively charged spray is formed by a flow of 0.6 mL min−1 (1:100 split) when separated compounds leave the capillary from the auxiliary liquid system with a nebulization pressure of 10 psig with nitrogen and a capillary voltage of 3500 V. A hot nitrogen flow of 10 mL min−1 at 200 °C was applied to dry the spray. |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
