Summary of Study ST002103
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001332. The data can be accessed directly via it's Project DOI: 10.21228/M8J980 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002103 |
| Study Title | NC HHEAR Hub Pilot Study within the HHEAR Consortium |
| Study Type | C18 Reversed-Phase Broad Spectrum Metabolomics |
| Study Summary | This data generation, and subsequent data analysis was conducted by the NC HHEAR Hub as part of a larger HHEAR Consortium Pilot Study. A goal of this study to compare the metabolite identifications and annotations provided by HHEAR Consortium Laboratories as well as non-HHEAR Consortium Laboratories. Urine samples were provided by the Duke HHEAR Hub. This included pools of 3 spot urines collected over 48 hours, which were selected based on maximum overlap of collected data on other exposure matrices (targeted PFAS panel (serum), passive air sampling, wristband data). Dr. Yuan Li of the NC HHEAR Hub had primary responsibility for the generation of the untargeted data. |
| Institute | University of North Carolina at Chapel Hill |
| Department | Nutrition Research Institute |
| Laboratory | Untargeted Resource Laboratory for the NC HHEAR Hub |
| Last Name | Li |
| First Name | Yuan |
| Address | Nutrition Research Institute, UNC-CH, 500 Laureate Way, Kannapolis, NC 28081 |
| yuanyli4@unc.edu | |
| Phone | 984-377-0693 |
| Submit Date | 2022-02-07 |
| Raw Data Available | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-21 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003437 | AN003438 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Voltage | Voltage |
Chromatography:
| Chromatography ID: | CH002540 |
| Chromatography Summary: | Reverse phase |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,3um) |
| Column Pressure: | 6000-10000 psi |
| Column Temperature: | 50 |
| Flow Gradient: | Time(min) Flow Rate %A %B Curve 1. 0 0.4 99.0 1.0 5 2. 1.00 0.4 99.0 1.0 5 3. 16.00 0.4 1.0 99.0 5 4. 19.00 0.4 1.0 99.0 5 5. 19.50 0.4 99.0 1.0 5 6. 22.00 0.4 99.0 1.0 5 |
| Flow Rate: | 0.4 ml/min |
| Injection Temperature: | 8 |
| Internal Standard: | Tryptophan-d5 |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Analytical Time: | 22 min |
| Randomization Order: | Randomized |
| Chromatography Type: | Reversed phase |
