Summary of Study ST002185
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001392. The data can be accessed directly via it's Project DOI: 10.21228/M8SH87 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002185 |
| Study Title | Lipidomic characterization of Jurkat-derived T cell line in which the chaperonin complex CCT has been partially silenced |
| Study Type | Untargeted Lipidomics |
| Study Summary | When the chaperonin complex CCT is partially silenced in Jurkat-derived T cell line J77 E61, we observe changes in the production of exosomes and in their composition. Thus, to explore the bases of these alterations and find possible new mechanisms of exosome biosynthesis regulation we characterized the lipidome content in cells where the chaperonin complex CCT was partially silent (CCT) and controls (CTRL). We analyzed 5 biological replicates containing 3 × 106 cells using LC-MS in positive and negative polarity mode. For quality control, 5 QCs samples and 4 blanks were also included in the analysis (total 19 samples and 2 groups). |
| Institute | Centro Nacional de Investigaciones Cardiovasculares Carlos III |
| Department | Proteomics and Metabolomics Unit |
| Laboratory | Metabolomics Lab |
| Last Name | Ferrarini |
| First Name | Alessia |
| Address | Calle de Melchor Fernández Almagro, 3, Madrid, Madrid, 28029, Spain |
| aferrarini@cnic.es | |
| Phone | 914 53 12 00 |
| Submit Date | 2022-06-03 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-06-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003578 | AN003579 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | mRP-Recovery C18 (100 × 0.5 mm,5um) | mRP-Recovery C18 (100 × 0.5 mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | LTQ-FT | LTQ-FT |
| MS instrument name | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | log Abundance | log Abundance |
Chromatography:
| Chromatography ID: | CH002646 |
| Chromatography Summary: | Lipidomics untargeted analysis was performed using an Ultimate 3000 HPLC equipped with Agilent mRP-Recovery C18 column (100 × 0.5 mm, 5 µm) thermostated at 55°C. Lipids were eluted at 100 mL/min using (A) water + 0.1% of formic acid and (B) acetonitrile with 0.1% formic acid. MS was operating in full scan mode from 70 to 1700 m/z at 60000 resolution in positive and negative polarity mode, in separate runs. |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | mRP-Recovery C18 (100 × 0.5 mm,5um) |
| Flow Rate: | 100 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
