Summary of Study ST002357
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001514. The data can be accessed directly via it's Project DOI: 10.21228/M80H6B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002357 |
| Study Title | Comparative lipidomics study of brain samples from deep-diving pinnipeds and terrestrial (non-diving) relatives |
| Study Summary | Brain samples from deep-diving pinnipeds (Cystophora cristata (n=10), Pagophilus groenlandicus (n=6)) were compared to terrestrial (non-diving) relatives (Mustela putorius furo (n=4), Mus musculus (n=9)). |
| Institute | University of Hamburg |
| Last Name | Martens |
| First Name | Gerrit Alexander |
| Address | Martin-Luther-King-Platz 3, 20146 Hamburg, Germany |
| gerrit.alexander.martens@uni-hamburg.de | |
| Phone | +49 40 42838-3934 |
| Submit Date | 2022-11-22 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-04-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003848 | AN003849 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Dionex Ultimate 3000 UPLC system (Dionex, Idstein, Germany) | Dionex Ultimate 3000 UPLC system (Dionex, Idstein, Germany) |
| Column | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker Daltonics maXis 3G | Bruker Daltonics maXis 3G |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH002849 |
| Chromatography Summary: | LC experiments were carried out using a RP C-18 column (150 × 2.1 mm, 1.7 μm, Phenomenex, Aschaffenburg, Germany) together with a Dionex Ultimate 3000 UPLC system (Dionex, Idstein, Germany). The mobile phase consisted of water (solvent A) and mixture of acetonitrile and isopropanol (1:3, v/v) (solvent B). Both eluents contained 10 mMol/L ammonium formate for measurements in positive ionization mode and 0.02% acetic acid for measurements in negative ionization mode. The column oven was set at 50°C and the flow rate was 300 µL/min. The gradient elution was as follows: 55% B (0-2 minutes); 55% to 75% B (2-4 minutes); 75% to 100% B (4-18 minutes); 100% B (18-23 minutes), 55% B (23-24 minutes); 55% B (24-27 minutes). For measurements in positive ionization mode, 2 µL of the sample extracts were injected while for analyzes in negative ionization mode 8 µL were used. The samples were analyzed in randomized order, with one blank sample and one QC sample being measured after each of the five animal samples. The autosampler in which the samples were stored during the measurement was set to 4° C. |
| Instrument Name: | Dionex Ultimate 3000 UPLC system (Dionex, Idstein, Germany) |
| Column Name: | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 50°C |
| Flow Gradient: | 55% B (0-2 minutes); 55% to 75% B (2-4 minutes); 75% to 100% B (4-18 minutes); 100% B (18-23 minutes), 55% B (23-24 minutes); 55% B (24-27 minutes) |
| Flow Rate: | 300 µL/min |
| Internal Standard: | 10 mMol/L ammonium formate for measurements in positive ionization mode and 0.02% acetic acid for measurements in negative ionization mode |
| Sample Injection: | 2 µL of sample extracts were injected for measurements in positive ionization mode and 2µl of the sample extracts were injected for analyzes in negative ionization mode |
| Solvent A: | 100% water |
| Solvent B: | 25% acetonitrile/75% isopropanol |
| Chromatography Type: | Reversed phase |
