Summary of Study ST002504
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001618. The data can be accessed directly via it's Project DOI: 10.21228/M8JT6D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002504 |
Study Title | Lipid droplets and peroxisomes are co-regulated to drive lifespan extension in response to mono-unsaturated fatty acids |
Study Summary | Dietary mono-unsaturated fatty acids (MUFAs) are linked to human longevity and extend lifespan in several species. But the mechanisms by which MUFAs extend lifespan remain unclear. Here we show that an organelle network involving lipid droplets and peroxisomes is critical for lifespan extension by MUFAs in C. elegans. MUFA accumulation increases lipid droplet number in fat storage tissues, and this is necessary for MUFA-induced longevity. Lipid droplet number in young or middle-aged individuals can predict remaining lifespan, consistent with a beneficial effect of lipid droplets on lifespan. Lipidomics datasets reveal that MUFA accumulation also modifies the ratio of membrane lipids and ether lipids, a signature predictive of decreased lipid oxidation. We validate that MUFAs decrease lipid oxidation in middle-aged individuals, and that this is important for MUFA-induced longevity. Intriguingly, the increase in lipid droplet number in response to MUFAs is accompanied by a concomitant increase in peroxisome number. Using a targeted screen, we identify genes involved in the co-regulation or uncoupling of this lipid droplet-peroxisome network. We find that induction of both organelles is optimal for lifespan extension. Our study uncovers an organelle network involved in lipid homeostasis and lifespan regulation and identifies a mechanism of action for MUFAs to extend lifespan, opening new avenues for lipid-based interventions to delay aging. For the manuscript only the conditions “control” and “ash-2 RNAi” are plotted |
Institute | Stanford University |
Last Name | Papsdorf |
First Name | Katharina |
Address | 290 Jane Stanford way, 94301 Palo Alto, CA, USA |
papsdorf@stanford.edu | |
Phone | +1 650 546 5366 |
Submit Date | 2023-02-03 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-03-20 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004120 | AN004121 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH003052 |
Chromatography Summary: | Lipid extracts were analyzed in a randomized order using an Ultimate 3000 RSLC system coupled with a Q Exactive mass spectrometer (Thermo Fisher Scientific) as previously described (PMID: 32612231). To identify complex lipids, isolated lipids were analyzed with untargeted lipidomics using liquid chromatography coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) (LC/MS). Lipids were separated using an Accucore C30 column 2.1 x 150 mm, 2.6 μm (Thermo Scientific, cat# 27826-152130) and mobile phase solvents consisted in 1 mM ammonium formate and 0.1% formic acid in 60/40 acetonitrile/water (A) and 1 mM ammonium formate and 0.1% formic acid in 90/10 isopropanol/acetonitrile (B). The gradient profile used was 30% B for 3 minutes, 30–43% B over 5 minutes, 43–50% B over 1 minute, 55–90% B over 9 minutes, 90-99% B over 9 minutes and 99% B for 5 minutes. Lipids were eluted from the column at 0.2 ml/min, the oven temperature was set at 30°C, and the injection volume was 15 μl. Autosampler temperature was set at 15°C to prevent lipid aggregation. |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
Column Temperature: | 30 |
Flow Gradient: | The gradient profile used was 30% B for 3 minutes, 30–43% B over 5 minutes, 43–50% B over 1 minute, 55–90% B over 9 minutes, 90-99% B over 9 minutes and 99% B for 5 minutes. |
Flow Rate: | 0.2 ml/min |
Sample Injection: | 5ul |
Solvent A: | 60% acetonitrile/40% water; 1mM ammonium formate; 0.1% formic acid |
Solvent B: | 90% isopropanol/10% acetonitrile; 1mM ammonium formate; 0.1% formic acid |
Chromatography Type: | Reversed phase |