Summary of Study ST002705
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001676. The data can be accessed directly via it's Project DOI: 10.21228/M82H8Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002705 |
| Study Title | Lipidomic Analysis of subventricular zone in adult mice. |
| Study Summary | Lipid metabolism plays an important role in neurogenesis. The present study was performed to investigate the role of serine racemase in lipid metabolism and adult neurogenesis. Conditional deletion of serine racemase in nestin precursor cells (nestin cre+) showed significant alterations in the different lipid classes in the subventricular zone of adult nestin-cre+ mice based on a lipidomics study. The raw data for the lipidomics study are presented. |
| Institute | Johns Hopkins University School of Medicine |
| Last Name | Roychaudhuri |
| First Name | Robin |
| Address | 655 West Baltimore Street |
| robinroychaudhuri@gmail.com | |
| Phone | 3108484336 |
| Submit Date | 2023-04-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-06-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004385 | AN004386 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker micrOTOF-Q II QTOF | Bruker micrOTOF-Q II QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | intensity | intensity |
Chromatography:
| Chromatography ID: | CH003290 |
| Methods Filename: | MS_Metadata-Roychaudhuri_et_al.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 42 |
| Flow Gradient: | 25 min gradient separation (0 min – 2% MPB, 250 μL/min; 3.0 min – 5% MPB, 250 μL/min; 8.0 min – 30% MPB, 250 μL/min; 21 min – 95% MPB, 230 μL/min; 25 min – 95% MPB, 230 μL/min); and 10 min re-equilibration (0 min – 95% MPB, 230 μL/min; 2 min – 5% MPB, 250 μL/min; 9.95 min – 5% MPB, 250 μL/min; 10 min – 5% MPB, 50 μL/min). |
| Flow Rate: | 250 microliter/min |
| Solvent A: | Nova MT MixA: 10 mM ammonium formate in 50:40:10 methanol/acetonitrile/water |
| Solvent B: | Nova MT MixB: 10 mM ammonium formate in 95:5 isopropanol/water |
| Chromatography Type: | Reversed phase |
