Summary of Study ST002980
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001856. The data can be accessed directly via it's Project DOI: 10.21228/M8T42G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002980 |
| Study Title | Water soluble metabolomics in mice upon loss of SHMT |
| Study Summary | The enzyme SHMT interconverts the amino acids serine and glycine as part of the folate cycle. To explore the role of SHMT in amino acid homeostasis, Mice were treated with a small molecule inhibitor of SHMT (SHIN2) or had Shmt2 genetically knocked-out in a liver specific manner. Serum and liver samples were collected and underwent LC-MS metabolomics analysis. |
| Institute | Princeton University |
| Last Name | McBride |
| First Name | Matthew |
| Address | Carl Icahn Lab, South Drive, Princeton, NJ 08544 |
| matthewmcbride@princeton.edu | |
| Phone | 8567457389 |
| Submit Date | 2023-11-14 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-12-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004897 | AN004898 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH003695 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters XBridge BEH Amide (100 x 2.1mm,2.5um) |
| Column Temperature: | 25°C |
| Flow Gradient: | 0 minutes, 85% B; 2 minutes, 85% B; 3 minutes, 80% B; 5 minutes, 80% B; 6 minutes, 75% B; 7 minutes, 75% B; 8 minutes, 70% B; 9 minutes, 70% B; 10 minutes, 50% B; 12 minutes, 50% B; 13 minutes, 25% B; 16 minutes, 25% B; 18 minutes, 0% B; 23 minutes, 0% B; 24 minutes, 85% B; 30 minutes, 85% B |
| Flow Rate: | 150 μl/min |
| Solvent A: | 95% water/5% acetonitrile with 20 mM ammonium acetate, 20 mM ammonium hydroxide, pH 9.4 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
