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MB Sample ID: SA028923
Local Sample ID: | Pool_4 |
Subject ID: | SU000582 |
Subject Type: | Cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | Suzuki, H., Moldoveanu, Z., Hall, S., et al. IgA1-secreting cell lines from patients with IgA nephropathy produce aberrantly glycosylated IgA1. J Clin Inv. 2008, 118, 629-639 |
Cell Primary Immortalized: | immortalized |
Cell Counts: | 1x10^7 cells / pellet |
Species Group: | Human |
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Collection:
Collection ID: | CO000576 |
Collection Summary: | An aliquot of cells (1 x 10^7) grown in suspension culture was transferred into a 15 mL conical tube containing 4x the culture volume of ice-cold 0.9% (w/v) NaCl. Cells were pelleted for 5 minutes at 1,500 rpm in a 4oC refrigerated centrifuge. Supernatant was removed, and the pellet was gently resuspended in 1 mL of ice-cold 0.9% (w/v) NaCl to wash and transfer into a 2 mL Lo-Bind microcentrifuge tube (Eppendorf, #022431102). Cells were pelleted for 3 minutes at 1,300 rcf in a 4oC refrigerated centrifuge. The entire supernatant was removed and pellets were snap-frozen in liquid nitrogen and immediately stored at -70°C. |
Sample Type: | Cell pellets |
Storage Conditions: | -80 C |
Tissue Cell Quantity Taken: | 1 x 10^7 |