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MB Sample ID: SA057013
| Local Sample ID: | ms6436-22 |
| Subject ID: | SU000987 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Collection:
| Collection ID: | CO000981 |
| Collection Summary: | We will perform a hyperinsulinemic-euglycemic clamp as previously described except that in this current proposal we will perform the study for 3 hours.(36) The goal for this infusion is to assess the effect of hyperinsulinemia on lysine and AAA kinetics and whether metformin changes the effect of hyperinsulinemia. We will collect samples every 10 min for glucose. 40% dextrose will be infused at a variable rate during the clamp to maintain euglycemia.(36) During the last hour of the clamp, 5 blood samples will be drawn for measurement of stable isotope tracer enrichment and amino acid concentrations. Previous studies have established that the combination of metformin and pioglitazone decrease lysine and AAA concentrations in overweight and obese adults with impaired fasting glucose or untreated diabetes.(7) Aim 3 is designed to establish whether changes in lysine and AAA flux are associated with changes in insulin sensitivity. Measurement of lysine and AAA flux will be repeated in 10 women with PCOS after three months of metformin and 10 women with PCOS randomized to receive no treatment for three months. Insulin sensitivity and other measures of glucose metabolism will be calculated from an oral glucose tolerance test using the oral minimal model as described below. |
| Sample Type: | Blood (plasma) |
