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MB Sample ID: SA057698
Local Sample ID: | 10_25_2016_islet_OC_14 |
Subject ID: | SU001003 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6N |
Age Or Age Range: | 4 weeks old (young) or 10-15 months old (old) |
Gender: | Male and female |
Animal Animal Supplier: | Charles River Labs |
Animal Feed: | standard chow diet (PicoLab Rodent Diet 20 pellets, #5053 |
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Collection:
Collection ID: | CO000997 |
Collection Summary: | Pancreata were digested using Liberase TL, and islets were purified using a Histopaque gradient followed by hand-picking under a dissecting microscope. Islets were allowed to recover overnight in RPMI 1640 media supplemented with 8 mM glucose, 10% FBS, 2 mM L-glutamine, 100 U/mL Pen/Strep, 1 mM sodium pyruvate, 10 mM HEPES, and 0.25 mg/mL amphoterecin B. |
Collection Protocol Filename: | Methods.pdf |
Sample Type: | Pancreatic Islets |