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MB Sample ID: SA086845
| Local Sample ID: | 11-Y27 |
| Subject ID: | SU001291 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Age Or Age Range: | 2 days after birth |
| Weight Or Weight Range: | above 1.3Kg at birth |
| Gender: | Female |
| Animal Animal Supplier: | Purdue University Animal Sciences Research and Education Swine facility |
| Animal Housing: | Education Swine facility |
| Animal Water: | ad libitum |
| Animal Inclusion Criteria: | Three to four gilts above 1.3Kg at birth were selected per litter from eight different sows which were monitored during parturition |
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Collection:
| Collection ID: | CO001285 |
| Collection Summary: | Blood samples were collected from gilts at 48 h postnatal. Gilts were euthanized approximately 48 h after birth using CO2 inhalation. Following euthanasia, skin of the abdominal and genital regions was cleaned thoroughly using 70% ethanol, and vaginal swabs were taken using a cytology brush (Puritan 2196 Removable Stiff Bristle Tip Brush; QuickMedical; Issaquah, WA) by inserting the tip of the brush into the vulva angled dorsally at 45˚. Once inserted to the base of the bristles, the brush was rotated 360˚ against the vaginal surface. Two consecutive swabs were collected from each animal, and swabs were placed in separate 15 ml sterile polypropylene conical tubes (Falcon™, Fisher Scientific, San Jose, California) and immediately placed on dry ice for transport. Samples were stored in a -80°C freezer until lipid extraction and analysis. Sows were milked during farrowing, and at 24 h after delivery of first piglet. For milk collection piglets were removed from the sow for approximately an hour and then 1 ml oxytocin (VetOne; Boise, ID; 20 USP/ml) was administered IM using a 20g x 1.5-inch needle into the vulva to stimulate milk letdown. Colostrum samples were collected manually from all teats and combined to create a uniform sample. Samples were stored until further analysis at -20°C |
| Sample Type: | Vaginal epithelium |
| Collection Method: | swab |
| Collection Location: | West Lafayette |
| Collection Frequency: | once |
| Collection Duration: | 1min |
| Storage Conditions: | -80℃ |
| Collection Vials: | Puritan 2196 Removable Stiff Bristle Tip Brush; QuickMedical; Issaquah, WA |
| Storage Vials: | 15 ml sterile polypropylene conical tubes (Falcon™, Fisher Scientific, San Jose, California) |
| Additives: | none |
