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MB Sample ID: SA094382
| Local Sample ID: | WT3 |
| Subject ID: | SU001379 |
| Subject Type: | Bacteria |
| Subject Species: | Salmonella enterica |
| Taxonomy ID: | 28901 |
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Collection:
| Collection ID: | CO001374 |
| Collection Summary: | Ten biologically independent cultures each of wild-type (DM9404) and ridA mutant (DM3480) strains were grown overnight in NB medium at 37 °C and used to inoculate (1% inoculum) 250 mL minimal glucose medium in 500 mL non-baffled flasks. Flasks were randomly arranged in an Innova®44 incubator and cultures were allowed to grow 16 h shaking at 180 RPM and 37 °C. Cultures were cooled on ice 5 min and then harvested by centrifugation at 7,000 x G for 10 min at 4 °C. The supernatant was decanted, pellets were resuspended in 10 mL ddH2O and transferred to sterile 15 mL conical tubes in which they were pelleted at 7,000 x G 10 min at 4 °C. Final supernatant was decanted and pellets were frozen in liquid nitrogen and stored at -80 °C prior to cell extractions. |
| Sample Type: | Bacterial cells |
| Storage Conditions: | -80℃ |
