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MB Sample ID: SA099808
| Local Sample ID: | BOL3_NEG C |
| Subject ID: | SU001445 |
| Subject Type: | Plant |
| Subject Species: | Quercus ilex |
| Taxonomy ID: | 58334 |
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Collection:
| Collection ID: | CO001440 |
| Collection Summary: | Sample collection: Mature acorns from holm oak (Quercus ilex L. subsp.ballota [Desf.] Samp.) were collected from four different trees located in Aldea de Cuenca (province of Cordoba, Andalusia, Spain). All acorns were picked at the optimal harvest maturity window on the same day. Sorting, disinfection, and storage of healthy acorns were conducted according to Bonner & Vozzo, (1987). Acorn flour preparation: Healthy acorns (20 units per tree) were scarified with a knife by making transversal and longitudinal cuts, thus permitting the pericarp to be rapidly removed. Flour was prepared by seed (without seed coat) grinding with liquid nitrogen in a blade mill (IKA Dry Mill Basic A10) until a powder was obtained (Valero Galván, Jorrín Novo, Cabrera, et al., 2012). Flour was lyophilized and then macerated in a mortar until a fine powder was obtained. Samples were stored at 4 ºC in within a desiccator, in darkness, until NIRS analysis or metabolite extraction. |
| Sample Type: | Acorns |
| Storage Conditions: | Room temperature |
