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MB Sample ID: SA138175
Local Sample ID: | 30 |
Subject ID: | SU001710 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Collection:
Collection ID: | CO001703 |
Collection Summary: | MCAD-/- mice were re-derived as germ-free (GF) for the purpose of this study. MCAD-/- mice or MCAD+/+ controls were maintained in sterile gnotobiotic isolators or cages with HEPA-filtered air supply. Urine from GF MCAD-/- and MCAD+/+ mice was collected under sterile conditions. Seven days subsequent, mice were mono-colonized with wild-type C. sporogenes (Cs) by gavage of 200 µL saturated overnight culture. Urine was collected one week after colonization. C. sporogenes colonization was verified with serial dilution plating. Urine samples were immediately frozen on dry ice and stored under -80 oC until analysis. |
Sample Type: | Urine |
Storage Conditions: | -80℃ |