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MB Sample ID: SA150508
Local Sample ID: | DRB18invivo_9 |
Subject ID: | SU001715 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Age Or Age Range: | 4 weeks mice |
Gender: | Male |
Animal Animal Supplier: | Jackson Laboratory |
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Collection:
Collection ID: | CO001708 |
Collection Summary: | A549 lung cancer xenograft tumors were obtained after sacrificing the mice. The tumors were then prepared according to the protocol described and metabolomics analysis was performed. |
Sample Type: | Tumor cells |
Collection Method: | Tumors were homogenized in a Beadbud homogenizer (Benchmark scientific) in a mixture of LC-MS grade ice-cold methanol and water (1:1; v/v). The supernatant was collected and sonicated in a water bath incubator for 15 minutes, followed by centrifugation at 13,000 rpm for 10 minutes and collection of the supernatant. |
Collection Location: | Homogenization vials |
Collection Frequency: | Once |
Collection Duration: | 4-5 minutes |
Volumeoramount Collected: | 1ml |
Storage Conditions: | -80℃ |
Collection Vials: | Polypropylene 1.5 ml tubes |
Storage Vials: | Polypropylene 1.5 ml |