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MB Sample ID: SA164654
| Local Sample ID: | StrainsMetabs_AJ_34 |
| Subject ID: | SU001852 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 ; PWK/PhJ ; WSB/EiJ ; NZO/HILtJ ; A/J ; 129S1/SvImJ ; CAST/EiJ ; NOD/ShiLtJ |
| Age Or Age Range: | 8-12 weeks |
| Gender: | Female |
| Animal Animal Supplier: | JAX (except C57BL/6, who came from Charles River) |
| Animal Housing: | standard |
| Animal Light Cycle: | standard |
| Animal Feed: | TEKLAD global |
| Animal Water: | standard |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001845 |
| Collection Summary: | See protocol document for full study details. Between 3 and 5 infected mice of each strain were euthanized each day from days 3-12 post-infection for cross-sectional analysis. Because the WSB/EiJ strain experiences delayed peak infection severity relative to the other mouse strains in this study, 3 or 4 WSB/EiJ mice were euthanized each day from days 3-17 post-infection for cross-sectional analysis. For each mouse strain, 2 uninfected control animals were euthanized at baseline and generally on odd-numbered days between days 3-12 or 3-17 for WSB/EiJ mice. Euthanasia was performed using carbon dioxide asphyxiation in accordance with Stanford University and APLAC guidelines for humane euthanasia. Following euthanasia, blood was collected via cardiac puncture using 25Gx5/8IN tuberculin syringes (Fisher Scientific 14-841-34). Syringes were primed by filling the syringe barrel with 0.5M EDTA, pH 8.0 anticoagulant and dispensing all but 50uL. Collected blood was stored on ice in 1.5mL Eppendorf tubes for 15-45 minutes before spinning at 1,000xg at 4 degrees C for 5 minutes in a tabletop centrifuge. Plasma was frozen at -80C immediately, and thawed/re-frozen once to aliquot for downstream cytokine, metabolite, and liver enzyme analyses. 100uL of plasma was shipped to Metabolon (https://www.metabolon.com/, Durham, NC, USA), which performed a combination of gas and liquid chromatography with mass spectrometry (GC/LC-MS). Compounds were identified by comparing sample peaks to an internal Metabolon library of known and unknown compounds. Raw peak values were obtained using area-the-curve. Additional data normalizations were performed by Metabolon to account for sample dilutions and day-to-day variation in instrument performance. |
| Collection Protocol Filename: | strainsmetabs_methods_for_mw.docx |
| Sample Type: | Blood (plasma) |
