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MB Sample ID: SA174903

Local Sample ID:bdivrou_mero_1_met_2
Subject ID:SU001955
Subject Type:Other organism
Subject Species:Babesia divergens
Taxonomy ID:32595
Genotype Strain:Rouen 1986

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Collection:

Collection ID:CO001948
Collection Summary:Merozoites were isolated from B. divergens infected erythrocite (iRBC) cultures at 40% parasitemia. The content from iRBC culture flasks was transferred to Falcon tubes and spinned at 600 x g and 4 ºC for 5 min. Then, supernatants were filtered once using 5 μm filters and twice using 1.2 μm filters (Versapor membranes) with the help of a 1 mL syringe. Subsequently, the resulting volume was spinned on a Falcon tube at 2000 x g and 4 ºC for 2 min. The supernatants were discarded, and merozoite-containing pellets were placed on ice.
Sample Type:merozoites isolated from RBC
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