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MB Sample ID: SA174903
Local Sample ID: | bdivrou_mero_1_met_2 |
Subject ID: | SU001955 |
Subject Type: | Other organism |
Subject Species: | Babesia divergens |
Taxonomy ID: | 32595 |
Genotype Strain: | Rouen 1986 |
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Collection:
Collection ID: | CO001948 |
Collection Summary: | Merozoites were isolated from B. divergens infected erythrocite (iRBC) cultures at 40% parasitemia. The content from iRBC culture flasks was transferred to Falcon tubes and spinned at 600 x g and 4 ºC for 5 min. Then, supernatants were filtered once using 5 μm filters and twice using 1.2 μm filters (Versapor membranes) with the help of a 1 mL syringe. Subsequently, the resulting volume was spinned on a Falcon tube at 2000 x g and 4 ºC for 2 min. The supernatants were discarded, and merozoite-containing pellets were placed on ice. |
Sample Type: | merozoites isolated from RBC |