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MB Sample ID: SA185792
| Local Sample ID: | 20181211-MMV693183B-2.4-2 |
| Subject ID: | SU002066 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | 3D7 |
| Cell Counts: | 1*10^8 |
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Collection:
| Collection ID: | CO002059 |
| Collection Summary: | Parasites were magnetically purified at the early trophozoite stage from cultures of 5-10% parasitemia. Samples were generated with 5 mL of media containing 1*10^8 cells (either parasites or human red blood cells) for the treatment condition. Excess media was removed and the cells were centrifuged and washed once with PBS. Metabolism was quenched with 90% methanol containing 0.25 micromolar of universally labeled C-13, N-15 aspartate. The samples were centrifuged and the metabolites in the methanol were collected and transferred to a new tube to be dried with nitrogen. |
| Collection Protocol Filename: | Metabolite_Extraction_for_LCMS_2017.pdf |
| Sample Type: | Cultured cells |
| Collection Location: | Millenium Science Complex, University Park, Pennsylvania |
| Storage Conditions: | -80℃ |
| Collection Vials: | 1.5 mL eppendorf |
| Storage Vials: | 1.5 mL eppendorf |
| Collection Tube Temp: | On ice |
| Tissue Cell Quantity Taken: | 1x10^8 cells per sample in 1 mL total volume |
