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MB Sample ID: SA203982

Local Sample ID:CID3
Subject ID:SU002204
Subject Type:Fish
Subject Species:Danio rerio
Taxonomy ID:7955

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Collection:

Collection ID:CO002197
Collection Summary:zebrafish larvae were treated with DMSO or CID661578 for 24 hours prior to global metabolomics analysis (n=6). Metabolites were extracted from pools of 10 zebrafish larvae at 5 dpf using a 80% methanol/water-based extraction method. Detailed metabolite extraction was described previously using 80% methanol/water as solvent (PMID: 24410464, PMID: 24894601, PMID: 25795660) and dry pellets were stored in -80 °C freezer until ready for LC-MS analysis. Pellets were reconstituted into 30 μL of sample solvent (water:methanol:acetonitrile, 2:1:1, v/v), and 5 μL was injected into the LC-MS.
Sample Type:new
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