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MB Sample ID: SA207379
| Local Sample ID: | H12121_45_02 |
| Subject ID: | SU002247 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002240 |
| Collection Summary: | After 3 days or 4 weeks of either HFD or CD feeding, each mouse was administrated a 1g/kg U-13C fructose oral gavage. Mice were anesthetized with 2% isoflurane, and their abdominal cavities were opened at the specified time. Abdominal tissues were displaced to identify the portal vein, and a 27G needle was used to immediately collect portal vein blood samples (~100 µL). Immediately after, cardiac blood was collected from the right ventricle using a 25G needle (~1 mL). The jejunum, pancreas, and liver tissues were quickly resected and snap-frozen in liquid nitrogen. Blood samples were placed on ice in the absence of anticoagulant for 20 minutes and centrifuged at 16,000 × g for 10 minutes at 4 °C to separate the serum and plasma fractions of each sample. Serum and tissue samples were kept at −80 °C until further analysis. |
| Collection Protocol Filename: | cwwong_methods.pdf |
| Sample Type: | Tissue |
