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MB Sample ID: SA208945
| Local Sample ID: | CR2-01_3_1_730 |
| Subject ID: | SU002259 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | MCF-7 |
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Collection:
| Collection ID: | CO002252 |
| Collection Summary: | Around 2x106 MCF-7 cells were plated per flask and then separately treated with SIMR3058 or SIMR3066 compounds for 12 hrs to perform proteomic analysis; the uniform number of cells per flask for each sample was to avoid the effect of variable cell number on the outcome of the specific experiment. A volume of 1 mL of the extraction solvent (methanol + 0.1% formic acid) was added to the cells, which quenched cellular metabolic activity. The cells were vortexed for 2 min to ensure the quantitative extraction of metabolites and stored in ice for 1 hr. After this, the insoluble cell matrices were subjected to intermittent ultrasonication using the COPLEY sonicator (QSONICA SONICATOR, USA) under 30% amplifier and for 30 seconds with an ice bath employed throughout the process. Following that, cells debris were centrifuged (15000 rpm, 10 min, -4 °C), and the supernatants were collected and processed for proteomics and metabolomics analysis. |
| Sample Type: | Breast cancer cells |
