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MB Sample ID: SA209226
| Local Sample ID: | 2020-08-17_Zang-43_Box2Redo-QC(I)#3_POS |
| Subject ID: | SU002264 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | 10-week and 24-month |
| Gender: | Male |
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Collection:
| Collection ID: | CO002257 |
| Collection Summary: | Wild-type (WT) C57BL/6 mice were used in this study. Endotoxemia was induced in young (10-week) and aged (24-week) male mice by lipopolysaccharide (LPS). Based on published results as well as observations in our laboratory, male and female mice showed significantly different susceptibility to systemic symptoms in sepsis models. Thus, male but not female mice were chosen for the experiments presented in this report. LPS was administered intraperitoneally (i.p.), and mice were weighed individually to determine the exact amount of LPS (MilliporeSigma, Burlington, MA; catalog number L3012) required to achieve the doses indicated in the figure legends. Sterile endotoxin-free PBS was used as a vehicle control in sham groups. In some experiments, Beclin-1-activating peptide (TB peptide), synthesized according to a published sequence24 by NonoPep (Shanghai, China), was administered i.p. at a dose of 16 mg/kg in 100μl of PBS 30 minutes post LPS-challenge. Heart tissue was harvested 18 hours post LPS challenge. |
| Collection Protocol Filename: | Zang_Protocol.docx |
| Sample Type: | Heart |
| Storage Conditions: | -80℃ |
