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MB Sample ID: SA210716
| Local Sample ID: | CR02_055 |
| Subject ID: | SU002285 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002278 |
| Collection Summary: | 5 x 104 Fh1fl/fl and 1 x 105 Fh1-/-CL1 and Fh1-/-CL19 cells were plated the onto 6-well plate and reverse transfected with siRNA. Before extraction, cells were counted using a separate counting plate. After that, cells were washed at room temperature with PBS twice and then kept on cold bath with dry ice and methanol. Metabolite extraction buffer (50% methanol, 30% acetonitrile, 20% ultrapure water, 5 µM final concentration valine-d8) was added to each well following the proportion 1×106 cells/0.5 ml of buffer. Plates were kept at −80°C and kept overnight. The following day, the extracts were scraped and mixed at 4°C for 15 min. After final centrifugation at max speed for 10 min at 4°C, the supernatants were transferred into LC-MS vials. |
| Sample Type: | Cultured cells |
