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MB Sample ID: SA212336
| Local Sample ID: | MS48-14 |
| Subject ID: | SU002313 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Collection:
| Collection ID: | CO002306 |
| Collection Summary: | 2x105 cells were plated onto 6-well plates in Plasmax media (5 replicates for each cell type). The day after, the medium was replaced with fresh one containing 15N-leucine and 15N-isoleucine for 27h. Before extraction, cells were counted using CASY cell counter (Omni Life Sciences) using a separate counting plate. After that, cells were washed at room temperature with PBS twice and then kept in a cold bath with dry ice and methanol before adding the metabolite extraction solution. |
| Sample Type: | Cultured cells |
