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MB Sample ID: SA212935
| Local Sample ID: | 109780 |
| Subject ID: | SU002320 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Collection:
| Collection ID: | CO002313 |
| Collection Summary: | For metabolite collection, plates containing 0.2-0.4 million cells per well were gently washed with 75mM ammonium carbonate buffer pH 7.4 at room temperature, transferred on ice, and metabolites were extracted with 80:20 ice-cold MeOH:H2O solution. Cells were scraped off and samples were collected in tubes, then snap frozen in liquid nitrogen to stop all metabolic reactions. |
| Sample Type: | U2OS cells |
