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MB Sample ID: SA218461
Local Sample ID: | WT3R |
Subject ID: | SU002366 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Collection:
Collection ID: | CO002359 |
Collection Summary: | Mouse colonies were bred at the CNIC under specific pathogen-free conditions and on C57BL/6 background. Tfamf/f (Larsson et al., 1998) mice were kindly provided by Nils-Göran Larsson (Max Planck Institute for Biology of Ageing, Cologne, Germany). All floxed mouse lines were crossed with CD11cCre mice (Caton et al., 2007). Mice were group-housed, have not been used in previous procedures and were fed standard chow. Littermates of the same sex were randomly assigned to experimental groups. Male and female mice were used for all experiments. Mice with 6–10-weeks (adult) were used for the experiment. Bronchoalveolar lavage (BAL) was performed by inserting a venal catheter (BD) into the trachea and 3-10 washes with 0.3-1 ml FACS buffer to harvest BAL cells. |
Collection Protocol Filename: | Protocol_AM.pdf |
Sample Type: | Bronchoalveolar lavage |
Collection Method: | Bronchoalveolar lavage (BAL) was performed by inserting a venal catheter (BD) into the trachea and 3-10 washes with 0.3-1 ml FACS buffer to harvest BAL cells. |
Storage Conditions: | -80℃ |