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MB Sample ID: SA237861
| Local Sample ID: | REH-ACM-MTX-3 |
| Subject ID: | SU002472 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Subject Comments: | Human B-ALL cell line (REH and RCH-AcV) were a generous gift from Dr. Christopher Porter at Emory University |
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Collection:
| Collection ID: | CO002465 |
| Collection Summary: | Human B-ALL cell lines (REH and RCH-AcV) were cultured for 24 hours in UCM, SCM, or ACM. Additionally, MTX-treated human B-ALL cells were cultured for an additional 12 hours of treatment with MTX (50nM for REH and 70 nM for RCH-AcV). This timepoint was chosen due to our prior work in this system to avoid submitting mostly apoptotic cells for analysis [18]. After the prescribed time, leukemia cells were harvested for analysis. Notably, cell viability at the time of harvest exceeded 85% in each culture (n=3 biological replicates). Cell viability was measured using cell a Bio-Rad cell counter (cat#TC20, Bio-Rad, Hercules, CA) and over 1 million viable cells were collected and washed with 1X cold PBS (pH7.4). Cell pellets were snap-frozen in dry ice and stored at -80oC until samples were submitted to our core facility. |
| Sample Type: | B cells |
| Storage Conditions: | -80℃ |
