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MB Sample ID: SA240125
| Local Sample ID: | #744_mito |
| Subject ID: | SU002492 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
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Collection:
| Collection ID: | CO002485 |
| Collection Summary: | Mitochondria were isolated by differential centrifugation. Freshly harvested renal cortex (50 mg) was finely minced and gently homogenized with glass Teflon tissue grinders in 2 ml ice-cold isolation medium, pH 7.2 (70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1mM EGTA). The homogenate was centrifuged at 800 g for 5 min at 4C and the resulting supernatant was centrifuged at 8,000 g for 10 min at 4C. After washing with 0.5 ml ice-cold isolation buffer, the mitochondrial pellet was resuspended in 200 l ice-cold isolation medium. Total protein was determined by the bicinchoninic acid method according to the manufacturer’s instructions (BCA Protein Assay Kit, Pierce-Thermo Fisher Scientific, Melbourne, Australia). |
| Sample Type: | Mitochondria |
