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MB Sample ID: SA241941
| Local Sample ID: | F2-27-09 |
| Subject ID: | SU002502 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
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Collection:
| Collection ID: | CO002495 |
| Collection Summary: | The human embryonic kidney 293T (HEK293T) and K562 cells were maintained in Iscove's modified Dulbecco’s medium (IMDM; Sigma), human THP1 cells were maintained in Roswell Park Memorial Institute medium (RPMI; Lonza). All media were supplemented with 10% fetal bovine serum (FBS; Gibco), penicillin (100 IU/ml), streptomycin (100 µg/ml) and 2% glutamine. A549 and Vero E6 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Sigma) supplemented with 10% fetal bovine serum (FBS; Gibco), 1% Non-essential amino acid (NEAA, Gibco), 1% Sodium Pyruvate (Gibco). Calu3 were cultured in Dulbecco's Modified Eagle Medium (DMEM, Sigma) supplemented with 20% fetal bovine serum (FBS; Gibco), 1% Non-essential amino acid (NEAA, Gibco), 1% Sodium Pyruvate (Gibco) and 2% GlutaMAX (Gibco). All cells were maintained in a 5% CO2 humidified atmosphere at 37°C. A549-hACE2 cell lines were generated by lentiviral transduction. Lentiviral vectors were produced following a standard procedure based on calcium phosphate co-transfection with 3rd generation helper and transfer plasmids. Primary cells Human CD34+ HSPC were isolated through positive magnetic bead selection according to manufacturer’s instructions (Milteney) from umbilical cord blood or from mononuclear cells collected upon informed consent from healthy volunteers according to the Institutional Ethical Committee approved protocol (TIGET01). Otherwise, CB, bone marrow (BM) or G-CSF mPB-CD34+ cells were directly purchased from Lonza or Hemacare. All cells were maintained in a 5% CO2 humidified atmosphere at 37°C. |
| Sample Type: | Cultured cells |
