Return to study ST002421 main page
MB Sample ID: SA242371
| Local Sample ID: | 20191130_neg_rep3_WT_a |
| Subject ID: | SU002510 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | HEK293T |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO002503 |
| Collection Summary: | Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and 100 units/mL penicillin and streptomycin. Cells were maintained in a humidified, 5 % CO2 atmosphere at 37°C. The CRISPR-Cas9 gene editing system was used to generate UBXD8 knockout cell lines in HEK293T cells. Cells were grown in 6-well plates for lipidomics. Cells were washed with PBS, scraped into cold 50% methanol, centrifuged, and the cell pellets were frozen at -80˚C. Next, cells were resuspended in cold 50% methanol (1mL) and transferred to glass vials. Chloroform was added (0.5mL) and the mixture was gently vortexed and centrifuged at 1,000x g for 5 min at 4˚C. Lipids were transferred to a clean glass vial using a glass Hamilton syringe. Lipids were extracted twice using chloroform prior to being dried under nitrogen gas. Samples were normalized according to protein concentration when resuspended in a 1:1:1 solution of methanol:chloroform:isopropanol prior to mass spectrometry (MS) analysis. |
| Sample Type: | Epithelial cells |
