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MB Sample ID: SA244721
| Local Sample ID: | PA CP27_1_25_1_972 |
| Subject ID: | SU002536 |
| Subject Type: | Bacteria |
| Subject Species: | Pseudomonas aeruginosa |
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Collection:
| Collection ID: | CO002529 |
| Collection Summary: | The Department of Wildlife and National Parks (PERHILITAN), Malaysia, allowed use of crocodile material. Additionally, the use of animals was approved by Sunway Research Ethics Committee, Sunway University, Malaysia (SUNREC 2019/023). A convention on international trade in endangered species (CITES) of wild fauna and flora registered crocodile farm, provided the saltwater crocodile, Crocodylus porosus. Management of crocodile including anesthesia and dissection of the internal organs were carried out by qualified personnel at the farm who routinely perform these procedures. Additionally, we also confirmed that all the experiments were carried out in agreement with appropriate protocols and guidelines as formerly defined (Akbar et al., 2019a). The whole gut was removed aseptically and culturable bacteria were isolated using sterile cotton swabs (Akbar et al., 2019b). Next, the culture were streaked on blood agar plates. The plates were incubated for overnight at 37°C with 5% CO2 and 95% humidity. Several bacterial species were observed and further differentiated based on their colony shape, appearance, colour and texture blood agar plates. Unlike bacterial colonies were sub-cultured on nutrient agar plates and incubated for overnight at 37°C. Finally, bacterial identification was done using microbiological as well as 16S rRNA gene amplification and sequencing (Akbar et al., 2019a). |
| Sample Type: | Bacterial cells |
