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MB Sample ID: SA248861
| Local Sample ID: | AG-23-TC-6 |
| Subject ID: | SU002579 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
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Collection:
| Collection ID: | CO002572 |
| Collection Summary: | C57BL/6 mice were orthotopically implanted with PK5L1940 cells. 8 Gy RT was administered 7 days post-implantation. PD1-IL2v and aCD25 dosed once per week beginning day 7 post-implantation. Spleens and lymph nodes of C57BL/6 mice were harvested 17 days post implantation and homogenized to a single cell suspension by mashing through a 70 uM cells strainer. For spleen processing, and the erythrocytes were lysed with ACK (ammonium-chloride-potassium) lysis buffer for 5 min at RT. CD8 T cells were sorted with a CD8-negative selection Stemcell isolation kit following manufacturer instructions. |
| Sample Type: | T-cells |
