Return to study ST002510 main page
MB Sample ID: SA252699
| Local Sample ID: | PTJ0108_2 |
| Subject ID: | SU002610 |
| Subject Type: | Bacteria |
| Subject Species: | Eggerthella lenta |
| Taxonomy ID: | 84112 |
| Genotype Strain: | various (see sample table) |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO002603 |
| Collection Summary: | For the comparative strain metabolomics experiment, 96-well polypropylene deep well plates were prepared with 800μL of fresh media in each well. Starter cultures and inocula for 29 isolates of Eggerthella lenta and 1 isolate of Eggerthella sinensis [26] were prepared as described above for growth assays, except without final dilution, and 80 μL was used to inoculate wells, leaving a blank well in between every culture well to prevent cross-contamination. After 72 hours, OD600 measurements were taken, plates were centrifuged, and supernatants were collected. Plates were centrifuged at 1,928 rcf at 4°C for 8 minutes, after which supernatants were collected into fresh polypropylene tubes or plates, sealed, and flash-frozen in liquid nitrogen. |
| Sample Type: | Bacterial culture supernatant |
| Collection Frequency: | single time point (72 hours) |
| Storage Conditions: | -80℃ |
