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MB Sample ID: SA252990
| Local Sample ID: | Serum_GF_26 |
| Subject ID: | SU002613 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 6-10 weeks |
| Gender: | Male |
| Animal Animal Supplier: | University of California, San Francisco Gnotobiotics core facility |
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Collection:
| Collection ID: | CO002606 |
| Collection Summary: | Sample collection was performed as described in doi.org/10.1016/j.chom.2021.11.001 . Briefly, C57BL/6J mice (males, ages 4-8 weeks) were obtained from the University of California, San Francisco Gnotobiotics core facility (gnotobiotics.ucsf.edu) and housed in Iso positive cages (Tecniplast). Mice were colonized via oral gavage with E. lenta monocultures (109 CFU/mL, 200 μl gavage, n=6 E. lenta DSM 2243, n=5 E. lenta DSM 15644, n=4 E. lenta AB12n2) or control media (n=5 GF). Colonization was confirmed via anaerobic culturing and/or qPCR for an E. lenta specific marker (elnmrk1) [10,26]. Mice were colonized for 2 weeks prior to sacrifice and sample collection. Serum samples were collected. |
| Sample Type: | Blood (serum) |
| Collection Frequency: | single time point (2 weeks after colonization) |
| Storage Conditions: | -80℃ |
