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MB Sample ID: SA254724
| Local Sample ID: | 20221116_QE2_JAS_Job2640_83 |
| Subject ID: | SU002631 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002624 |
| Collection Summary: | 100,000-150,000 cells were seeded in 2mL of culture medium in six-well plates with 3 technical replicates per condition per time point and allowed to attach overnight. The following day (day 1), cells were washed twice with 2mL PBS. They were then given 2mL of media, either TIFM or RPMI. An unspent media sample was collected at this time as well and stored at -80 °C. Cell number on day 1 was measured using a Vi-CELL XR Cell Viability Analyzer (Beckman Coulter). 24h later (day 2), 1mL of spent media from cells was collected, centrifuged and stored at -80 °C. Cell number was counted again. Quantification of metabolite levels in unspent (day 1) and day 2 (conditioned media) cell culture media samples was performed. |
| Sample Type: | Cell culture media |
