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MB Sample ID: SA254928
| Local Sample ID: | 1-1_nontumor |
| Subject ID: | SU002634 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002627 |
| Collection Summary: | Brains were embedded in 5% wt. carboxymethyl cellulose (Millipore Sigma) in water and stored at -80 °C. Next, 20 µm thick sections were collected at -20 °C by using a CM1860 cryostat (Leica Biosystems). Superfrost Plus slides (Thermo Fisher Scientific) were used after cleaning with ethanol. Sections were dried under vacuum, stored at -80 °C until use, and thawed under vacuum immediately prior to analysis. For MALDI, 10 µm thick sections were collected on SiO2 passivated, indium tin oxide coated polished float glass slides (Delta Technologies Limited, Loveland, CO, USA). Serial 50 µm thick sections were collected for extraction and LC/MS analysis after dividing between tumor-containing and non-tumor hemispheres (as described below). |
| Sample Type: | Brain |
