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MB Sample ID: SA271300
| Local Sample ID: | R Control 2A-01_14_1_3231 |
| Subject ID: | SU002808 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Collection:
| Collection ID: | CO002801 |
| Collection Summary: | The human non-small cell lung cancer cell line (A549) utilized in this study was purchased from AmericanType Culture Collection (ATCC) (Manassas, USA). A doxorubicin-resistant A549 cell line was generated in our laboratory (Drug Design and Discovery Research Group, Pharmacology Laboratory, Sharjah Institute for Medical Research, University of Sharjah). Doxorubicin (Dox) and irinotecan were purchased from Sigma-Aldrich (Darmstadt, Germany) and Abcam (Cambridge, UK), respectively. A Dulbecco's Modified Eagle Medium/ Nutrient Mixture F-12 (1:1) (DMEM/F-12) medium was purchased from Gibco, Thermo Fisher Scientific (Massachusetts, USA). Fetal bovine serum (FBS), penicillin/streptomycin, trypsin, and trypan blue were purchased from Sigma-Aldrich (Darmstadt, Germany). For cell viability assay: MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reagent and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Darmstadt, Germany). Protease inhibitor cocktail tablets were purchased from Roche (Mannheim, Germany). Methanol (≥99.9%), acetonitrile (ACN) and deionized water, as well as LC-MS CHROMASOLV, were purchased from Honeywell (Seelze, Germany). Trifluoroacetic acid (TFA) was purchased from Sigma-Aldrich (Darmstadt, Germany) and acetonitrile (ACN) was purchased from Honeywell (Charlotte, USA). Lys-C endopeptidase was purchased from Fujifilm Wako Chemicals (Virginia, USA). For western blot analysis: primary antibodies against liver carboxylesterase 1/CES1 and β-actin were obtained from Abcam (Cambridge, UK) and Cell Signaling Technologies (Danvers, USA), respectively. Anti-rabbit IgG, Horseradish Peroxidase (HRP)-linked secondary antibody was purchased from Cell Signaling Technologies (Danvers, USA). Skimmed milk was purchased from Sigma-Aldrich (Darmstadt, Germany). For Enzyme-linked immunosorbent assay (ELISA): carboxylesterase 1 (CES1) specific activity assay kit purchased from Abcam (Cambridge, UK). |
| Sample Type: | Lung |
