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MB Sample ID: SA273548
| Local Sample ID: | 5B |
| Subject ID: | SU002819 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
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Collection:
| Collection ID: | CO002812 |
| Collection Summary: | Mice were bred and maintained in the animal facility of the University of Lausanne. All animal experiments were authorized by “Service de la consommation et des Affaires vétérinaires” (SCAV) of the Canton de Vaud in Switzerland. Cx3cr1CREERT2;MCT4flox mice were obtained by crossing the B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2)Litt/WganJ mice (Cx3cr1CREERT2; No: 021160, The Jackson Laboratory) with C57Bl/6.MCT4tm1flox mice (MCT4flox; kindly donated by Prof. Luc Pellerin). For specific experiments, the Cx3cr1CREERT2;MCT4flox line was further crossed with B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J mice (TdTomatoflox; No: 007914, The Jackson Laboratory). Mice were group-housed and kept in a 12 h day/night cycle, with food and water ad libitum, at 20-22°C. All the experiments were performed during the day cycle. Both male and female mice were analyzed, unless differently specified. For in vivo gene KO induction, tamoxifen (Sigma Aldrich) was prepared in 10% ethanol in corn oil at a concentration of 6mg/ml. The whole litter was injected intraperitoneally (i.p.) at P6 and P8 (75mg/kg). For biochemical and histological assessments, mice at P15 were terminally anesthetized with sodium pentobarbital diluted in saline (150mg/kg, i.p.) and perfused with cold HBSS (Life Technologies, 3ml/min). Upon brain collection, the hippocampus was dissected from the right hemisphere, immediately frozen in dry ice and stored at -80°C. |
| Sample Type: | Brain |
