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MB Sample ID: SA273963
| Local Sample ID: | 9 |
| Subject ID: | SU002831 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Collection:
| Collection ID: | CO002824 |
| Collection Summary: | Whole blood samples (1 mL) were obtained from animals via cardiac puncture, using a syringe with a 26-gauge needle, and placed in an EDTA treated tube. The blood was transferred to a 15 mL conical tube and diluted 2:1, sterile PBS: blood, and gently mixed. Lympholyte® Mammal Cell Separation media (Cedarlane Labs, product # CL5115) was gently added to the bottom of the blood solution and spun at 1400 rpm for 30 minutes in a refrigerated centrifuge. After centrifugation, layers were visualized, the PBMC layer (midlayer) was extracted and resuspended in a new tube. The isolated PBMC’s were washed using ~14mL sterile PBS, spun at 1800 rpm for 10 minutes, excess PBS was removed, cells were resuspended in 1-2mL for counting, and the final pellet was frozen in liquid nitrogen and stored at -80C. |
| Sample Type: | Mononuclear cells |
