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MB Sample ID: SA288725
| Local Sample ID: | RJ_27ZW1 |
| Subject ID: | SU002843 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
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Collection:
| Collection ID: | CO002836 |
| Collection Summary: | LV tissue from patients undergoing CABG was sampled from two or more pre-determined areas (as guided by CMR) on the beating heart during surgery: 1) a region of viable myocardium with inducible ischemia [‘ischemic’ biopsy]; 2) a region of remote myocardium with normal contractility, no qualitative evidence of inducible hypoperfusion on perfusion imaging, and without infarct pattern LGE [‘remote’ biopsy]. The a priori plan was to acquire paired biopsies, permitting high energy phosphate (HEP) quantification and LC-MS for each individual patient. Surgical discretion, however, resulted in some patients not undergoing paired tissue collection. Conversely, where deemed safe, extra tissue was acquired for single-nuclei RNA sequencing. The CABG biopsies were performed on the beating heart (prior to aortic cross-clamp, cardioplegia and hypothermia in the cases using cardiopulmonary bypass) with a Tru-Cut® needle or scalpel by the consultant cardiac surgeon. The majority of operations were performed off-pump without cardiopulmonary bypass. Samples were immediately clamp-frozen in theatre using a Wollenberg clamp (manufactured by Josh Firman, LMB Workshop, UK) which had been pre-cooled in liquid nitrogen until tissue acquisition. The clamps were then reopened, and the tissue rapidly transferred into Eppendorf tubes (pre-cooled in dry ice) before storage at -80ºC awaiting further analysis. |
| Collection Protocol Filename: | Protocol_AMBITION.pdf |
| Sample Type: | Heart |
