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MB Sample ID: SA326125
| Local Sample ID: | 10256_Diagnosis_Met |
| Subject ID: | SU003107 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Gender: | Female |
| Animal Housing: | Free-stalls |
| Animal Feed: | TMR |
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Collection:
| Collection ID: | CO003100 |
| Collection Summary: | All cows had uterine fluid collected at calving (first 24h after calving), and at diagnosis (day of metritis diagnosis). Briefly, cows’ cervix was stabilized by rectal palpation, the vulva was rinsed with alcohol 70% and dried with paper towels. Subsequently, a single-use plastic round-tip pipette (UterFlush pipettes, Van Beek) was introduced into the vagina at a 45° angle and manipulated through the cervix. A total of 50 mL of sterile saline solution (0.9% sodium chloride irrigation, Baxter) was infused into the uterine lumen using a 60-mL syringe (Covidien) attached to the end of the pipette. Uterine contents were homogenized, retrieved into the same 60-mL syringe, and transferred to a sterile 15-mL conical tube (VWR). After collection, tubes were placed on ice and transported to the laboratory within 2 hours. Once in the laboratory, uterine fluid samples were aliquoted into 2-mL microcentrifuge tubes (Eppendorf) and stored at -80 oC until essayed. One frozen uterine fluid aliquot was submitted to the University of California’s West Coast Metabolomics Center in Davis, CA for metabolome analysis. |
| Sample Type: | Uterine fluid |
