Return to study ST003066 main page
MB Sample ID: SA332020
| Local Sample ID: | 1699490 |
| Subject ID: | SU003181 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO003174 |
| Collection Summary: | This study utilized a previously reported twin study database comprising metabolomics and proteomics of erythrocytes in adult twins. The study was approved by the Human Subjects office of The University of Iowa Carver College of Medicine. Written informed consent was obtained from all participating subjects. Subjects qualified for participation by meeting criteria for autologous blood donation according to standard operating procedures of The University of Iowa DeGowin Blood Center. Five dizygotic (DZ) and 13 monozygotic (MZ) twin pairs participated in this study, but twin pairs were not required to donate samples concurrently. Standard health history and demographic information was obtained at the time of enrollment and informed consent. Whole venous blood (EDTA, Vacutainer® purple top blood collection tube, 8 ml) collected from participants prior to blood donation was centrifuged at 500 × g for 5 min, followed by removal of the plasma and buffy coat. RBCs were washed twice with cold isotonic saline solution. After washing, a 30 μl aliquot of the packed red blood cells (pRBCs) was removed for complete blood count (CBC) analysis (Sysmex XE-2100™ Automated Hematology System, Sysmex Corp). A 100 μl aliquot of pRBCs was lysed with 900 μl of nanopure water. Samples were thoroughly mixed and stored at −80 °C prior to proteomic and metabolomic analyses. |
| Sample Type: | Erythrocytes |
