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MB Sample ID: SA332488
| Local Sample ID: | Muscle_502 |
| Subject ID: | SU003191 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Gender: | Male |
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Collection:
| Collection ID: | CO003184 |
| Collection Summary: | C57BL/6 mice were housed North Carolina State University Biological Resources Facility with ad libitum access to food (Laboratory Rodent Diet 5001) and water on a 12-hour light/dark cycle. At the age of 8 weeks, mice were administrated metformin in their drinking water (1mg/ml) for 12 days. Before tissue and blood collection, mice were fasted for 5 hours (6am-11am). Mice were then anesthetized with isoflurane and sacrificed via cervical dislocation. Blood was collected into tubes containing EDTA anticoagulant via cardiac puncture. Plasma was obtained by centrifugation of whole blood at 1,500 g for 10 min at 4 °C and stored at -80 °C freezer. Tissues were immediately snap-frozen in liquid nitrogen, except that small intestines were rinsed with PBS and then the jejunum portion was stored in -80 °C freezer until further analysis. |
| Sample Type: | plasma, adipose, liver, muscle, heart, kidney, intestine, adipose |
