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MB Sample ID: SA333299
| Local Sample ID: | 02_008 |
| Subject ID: | SU003208 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Weight Or Weight Range: | 400g |
| Gender: | Male and female |
| Animal Animal Supplier: | Charles River |
| Animal Housing: | Double Housed |
| Animal Light Cycle: | Reverse Light Cycle |
| Species Group: | Sprague Dawley |
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Collection:
| Collection ID: | CO003201 |
| Collection Summary: | Brain samples were harvested and collected following transcardial perfusion with phosphate buffer (0.1 M, pH 7.4) 24 hr post-TBI. The perfused whole brains were rapidly removed, and flash frozen in an isopentane-methanol ice slurry. Pieces of parietal cortices (5 mm x 2 mm) were dissected from partially thawed brains by removing the subcortical structures including the majority of white matter and stored at -80° C in microcentrifuge tubes. The cortices were then transferred to liquid nitrogen and manually pulverized with a pestle and mortar submerged in liquid nitrogen and aliquoted in ~10-30 mg tissue samples. |
| Sample Type: | Brain |
