Summary of Study ST002969
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001848. The data can be accessed directly via it's Project DOI: 10.21228/M8V42S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002969 |
| Study Title | Polar metabolites in cecal tissue of mice treated with or without ampicillin and tributyrin |
| Study Summary | The chromatin landscape integrates diverse cellular signals to regulate genome structure and subsequent biological functions, partly through posttranslational modifications (PTMs) on histone proteins. Many donor molecules for histone PTMs are metabolites and are therefore impacted by cellular metabolism and environmental cues. In this study, we aimed to investigate how chromatin and cellular metabolism are linked in the intestine. One class of metabolites in intestinal lumen is short chain fatty acids (SCFAs), which are generated by the commensal microbiota. We found that select histone PTMs (including acetylation, butyrylation, and propionylation) are located in intestinal epithelial cells and are dependent on the presence of microbes. Histone butyrylation is associated with active gene expression and regulated by the metabolite tributyrin, which increases metabolites related to butyrate metabolism and induces specific metabolic gene programs. Together, these studies demonstrate a physiological setting in which previously uncharacterized histone acylations are dynamically regulated through metabolites and associated with gene expression. |
| Institute | Case Western Reserve University |
| Department | Biochemistry |
| Laboratory | Gates |
| Last Name | Gates |
| First Name | Leah |
| Address | Wood Building W456, 2109 Adelbert Road, Cleveland, Ohio 44106-4395 |
| leah.gates@case.edu | |
| Phone | 216-368-5572 |
| Submit Date | 2023-08-28 |
| Num Groups | 3 |
| Total Subjects | 15 |
| Num Females | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-12-04 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003075 |
| Collection Summary: | Intestine sections were extracted, cleaned of all feces, washed in cold PBS, and then flash-frozen in liquid nitrogen. |
| Sample Type: | Cecum |
