Summary of Study ST001657
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001062. The data can be accessed directly via it's Project DOI: 10.21228/M8F11N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001657 |
Study Title | E.coli K-12 treated by IPL_analysis of organic phase |
Study Summary | In this study, E.coli K-12 was treated by intense pulsed light (IPL) for 0-20 seconds. Then the organic/lipid phase of the cellular metabolome was extracted and submitted to untargeted LC-MS based metabolomic study. |
Institute | University of Minnesota |
Department | Food Science and Nutrition |
Laboratory | Nutritional Metabolomics |
Last Name | Chen |
First Name | Chi |
Address | 1334 Eckles Ave |
chichen@umn.edu | |
Phone | 6126247704 |
Submit Date | 2021-01-20 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2021-02-01 |
Release Version | 1 |
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Collection:
Collection ID: | CO001727 |
Collection Summary: | E. coli strain K-12 W3110 (ATCC 27325) was cultured on TSA agar medium. A single bacterial colony was picked from TSA agar medium, and then used to inoculate Luria-Bertani broth (EMD Millipore, Billerica, MA). After being incubated at 37 °C for 12 h on a rotary shaker set to 200 rpm, E. coli cells were harvested at an optical density (OD600) of 1, and then centrifuged at 7,500 × g for 10 min in 50 mL centrifuge tubes. After decanting the supernatant, the pellet was washed with phosphate buffered saline (PBS), and then re-suspended to the volume of bacterial culture for further treatment and analysis. |
Collection Protocol Filename: | Cell_culture_IPL_treatment_Sample_preparation_method.docx |
Sample Type: | Bacterial cells |