Summary of Study ST000228

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000185. The data can be accessed directly via it's Project DOI: 10.21228/M8BS32 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000228
Study TitleAnalysis of Dried Blood Spots by MALDI-MS
Study TypeAnalysis of Dried Blood Spots by MALDI-MS
Study SummaryConcentrations of cocaine (5,3,2,1.5,1,0.75,0.5,0.25,0.125, 0.062, and 0.031 ppm) were spiked into whole human blood for MALDI-MS analysis. A 10 uL volume of blood/analyte mixture was allowed to dry on Whatman Grade 2 filter paper. A 1ppm concentration Cocaine-d3 was used as an internal standard. Parameters of the MALDI system were optimized, including laser energy, number of laser shots, and matrix. The internal standard was added on top of the dried blood spot sample. Wide isolation tandem MS was used to generate a calibration curve.
Institute
University of Florida
DepartmentChemistry
LaboratoryChemistry Lab Building
Last NameDhummakupt
First NameElizabeth
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailemuffly@ufl.edu
Phone352-392-0515
Submit Date2015-07-16
Num Groups11
Total Subjects154
Study Comments11 concentrations were run as 7 samples/concentration for 2 days
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Uploaded File Size128 MB
Analysis Type DetailMALDI-MS
Release Date2017-10-03
Release Version1
Elizabeth Dhummakupt Elizabeth Dhummakupt
https://dx.doi.org/10.21228/M8BS32
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cocaine conentration (ppb)
SA0108271ppmCoc_1ppmCoc_intensity_AVG1000
SA010823125ppbCoc_1ppmCoc_intensity_AVG125
SA0108281.5ppmCoc_1ppmCoc_intensity_AVG1500
SA0108292ppmCoc_1ppmCoc_intensity_AVG2000
SA010824250ppbCoc_1ppmCoc_intensity_AVG250
SA0108303ppmCoc_1ppmCoc_intensity_AVG3000
SA01082131ppbCoc_1ppmCoc_intensity_AVG31
SA010825500ppbCoc_1ppmCoc_intensity_AVG500
SA0108315ppmCoc_1ppmCoc_intensity_AVG5000
SA01082262ppbCoc_1ppmCoc_intensity_AVG62
SA010826750ppbCoc_1ppmCoc_intensity_AVG750
Showing results 1 to 11 of 11
  logo